Formulation and antimicrobial activity evaluation of a 0.2% chlorhexidine canine mouthwash with essential oils

  • German L Madrigal Redondo Universidad Latina de Costa Rica
  • Daniela Gonzalez Corrales Universidad Latina de Costa Rica
  • Marianela Chavarria Rojas Universidad Latina de Costa Rica https://orcid.org/0000-0002-0507-6982
  • Tatiana Monge Quiros Universidad Latina de Costa Rica
  • Wendy Cruz Sibaja Universidad Latina de Costa Rica
  • Norman Rojas Campos Universidad de Costa Rica
Keywords: periodontitis, microemulsion, oregano, thyme, chlorhexidine, essential oil

Abstract

Background: Periodontal disease is the infection and inflammation of the gums, bones, and tissues involved in teeth support, and it is one of the most common diseases affecting pet dogs. Essential oils have shown antimicrobial activity against bacteria causing periodontal disease; therefore, they are considered potential therapeutic agents. Objectives: The main objective was to formulate and evaluate the antimicrobial activity of a 0.2% chlorhexidine canine mouthwash with essential oils. Methods: Three microemulsion formulations were obtained by constructing a pseudo-ternary phase diagram using the phase titration method. Different surfactant agents were evaluated, and hydrogenated castor oil was selected as the emulsifier agent. The antimicrobial activity of oregano essential oil (Origanum vulgare), thyme essential oil (Thymus vulgaris), and the three formulations were evaluated against Staphylococcus aureus, Streptococcus mutans, Streptococcus salivarius, and Escherichia coli. Results: Pure thyme and oregano essential oils showed higher antimicrobial activity than a 0,2% chlorhexidine solution. The formulations with essential oils plus chlorhexidine and chlorhexidine alone showed antimicrobial activity. The formulation containing only essential oils did not show antimicrobial activity. Conclusions: A canine mouthwash was formulated with chlorhexidine and thyme and oregano essential oil. Based on the evaluation of antimicrobial activity, two of the proposed formulations could be a therapeutic option to reduce the risk and prevent periodontal disease in canines.

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Author Biographies

German L Madrigal Redondo , Universidad Latina de Costa Rica

School of Pharmacy Universidad Latina de Costa Rica, Faculty of Pharmacy Universidad de Costa Rica

Daniela Gonzalez Corrales, Universidad Latina de Costa Rica

School of Pharmacy

Marianela Chavarria Rojas , Universidad Latina de Costa Rica

School of Pharmacy Universidad Latina de Costa Rica, Faculty of Pharmacy Universidad de Costa Rica

Tatiana Monge Quiros , Universidad Latina de Costa Rica

School of Pharmacy 

Wendy Cruz Sibaja , Universidad Latina de Costa Rica

School of Pharmacy 

Norman Rojas Campos , Universidad de Costa Rica

Faculty of Microbiology

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Published
2021-03-17
How to Cite
Madrigal Redondo G., Gonzalez Corrales D., Chavarria Rojas M., Monge Quiros T., Cruz Sibaja W., & Rojas Campos N. (2021). Formulation and antimicrobial activity evaluation of a 0.2% chlorhexidine canine mouthwash with essential oils. Vitae, 28(01). https://doi.org/10.17533/udea.vitae.v28n1a341703
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