Antioxidant and Acetylcholinesterase Inhibitor Potentials of the Stem Extract of Pternandra galeata

Authors

  • Suciati Suciati Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga, Surabaya 60115, East Java, Indonesia.
  • Dwiki Nur Inayah Center for Natural Product Medicine Research and Development, Institute of Tropical Diseases, Universitas Airlangga, Surabaya, 60115, East Java, Indonesia.
  • Aty Widyawaruyanti Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga, Surabaya 60115, East Java, Indonesia.
  • Rudiyansyah Rudiyansyah Department of Chemistry, Faculty of Mathematics and Natural Sciences, Tanjungpura University, Kubu Raya 78115, West Kalimantan, Indonesia.

DOI:

https://doi.org/10.17533/udea.vitae.v29n3a349983

Keywords:

Pternandra galeata, Alzheimer's disease, Acetylcholinesterase inhibitor, Antioxidant, Phenolic compounds

Abstract

Background: Pternandra galeata belongs to the family Melastomataceae. It is a native flowering plant in Borneo Island that serve as food for monkey habitat. There has been limited study on the medicinal and chemical properties of this plant. Objectives: We investigated the acetylcholinesterase inhibitory activity and evaluated the antioxidant activity of the ethanolic extract of Pternandra galeata stem. The total phenolic content in the sample was also determined. Methods: The acetylcholinesterase inhibitory assays were performed using Ellman’s method. Two different methods were used to evaluate the antioxidant activity of 2,2-diphenyl-1-picryl hydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. The total phenolic content was determined by the Folin-Ciocalteu method by employing gallic acid as a reference. Results: The ethanolic extract of the P. galeata stems inhibited the AChE enzyme with an IC50value of 74.62 ± 0.89 µg/mL. The sample exhibited antioxidant activity in the DPPH assay with an IC50 value of 20.21 ± 0.08 µg/mL and 7.68 ± 0.09µg/mL in the ABTS scavenging assay. The total phenolic content was 164.71 ± 3.33
mg GAE/g extract. ConclusionThe ethanolic extract of the P. galeata stem can be a promising cholinesterase inhibitor and antioxidant for treating Alzheimer’s disease.
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DPPH and ABTS radical scavenging effects of different concentrations of P. galeata extract

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Published

08-10-2022

How to Cite

Suciati, S., Inayah, D. N., Widyawaruyanti, A., & Rudiyansyah, R. (2022). Antioxidant and Acetylcholinesterase Inhibitor Potentials of the Stem Extract of Pternandra galeata. Vitae, 29(3). https://doi.org/10.17533/udea.vitae.v29n3a349983

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Natural Products

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