ANTIOPHIDIAN PROPERTIES EVALUATION OF ETHANOL EXTRACTAND FRACTIONS OBTAINED FROM Renealmia alpinia (Rottb) Mass (Zingiberaceae)CULTIVATED in vitro.
Keywords:Renealmia alpinia, cultures in vitro, fractions, Bothrops asper, antiophidian properties.
Plant extracts are a rich source of pharmacologically active molecules. Their application allows a tradicional
medicine approach to potential biological acitivities. This paper evaluates the inhibitory capacity
of ethanolic extracts of leaves and little roots and also fractions chromatographically fractions obtained
from Renealmia alpinia (Rottb) Mass, cultured in vitro, on the indirect hemolytic activity, proteolytic activity
and coagulant activity induced by the Bothrops asper venom. Indirect hemolytic activity is inhibited
to a greater extent by the fraction 7-8 (47.3 ± 2.20%) followed in order by the extracts from little roots
(32.6 ± 6.90%) and leaves (24.2 ± 4.43%). They came from in vitro and ex vitro leaves (16.2 ± 3.88%). The
proteolytic activity is largely inhibited by the leaves extracts in vitro and ex vitro without significant differences
between them. Little roots in vitro showed the highest neutralization effect on coagulant activity
(81.73 ± 9.949s). Proteolytic activity from Renealmia alpinia extracts on Bothrops asper venom is ruled out
since there are not changes in the electrophoretic pattern of the venom. The results make possible the
implementation of Renealmia alpinia as adjuvant for the treatment of ophidic accidents and sustain the
value of micropropagation for mass production of active components.
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