Prevalence of Anti-Leptospira spp. Antibodies among People with Occupational Exposure in Tolima Department

Authors

  • Blanca L. Guzmán Barragán National Institute of Health
  • Sandra M. Peña Perdomo Departmental Laboratory of Public Health, Secretariat of Health of Tolima
  • Roy F. Flórez Rojas University of Tolima
  • Diego F. Amado Gómez University of Tolima, Secretariat of Health of Tolima
  • Elkin M. Rodríguez Ruíz University of Tolima, Secretariat of Health of Tolima
  • Noel Verjan García University of Tolima

DOI:

https://doi.org/10.17533/udea.rfnsp.v34n2a04

Keywords:

leptospira, public health surveillance, health programs and plans, health evaluation

Abstract

Objective: to estimate the prevalence of IgM antibodies against Leptospira spp. Using the enzyme-linked immunosorbent assay (elisa) in a population at occupational risk from 8 municipalities of the Tolima department, Colombia. Methodology: blood samples were collected from 261 employees and analyzed with the elisa technique to detect IgM and anti-Leptospira spp. antibodies. This was followed by mat and serotyping. Results: a seroprevalence of 25.29% was estimated, with higher seroreactivity for individuals
working at slaughter plants (34.2%), collecting solid waste (27.1%) and those in contact with water and sewage waste (14.8%). Activity in slaughter plants was identified as a risk factor for Leptospira spp. (OR = 1.86). The serovars identified were L. Bratislava (16), Ballum (5), Tarassovi (3), Hebdomadis (2), Sejroe (2), and Icterhemorragiae (1). The municipality of Libano had the highest percentage of positivity (36.96%), followed by Espinal and Guamo with 28.57% each. Discussion: assessment of the current surveillance system for leptospirosis indicated deficient resources and health professionals who are lacking in terms of knowledge
regarding appropriate procedures, research on, diagnosis and reporting mechanisms for the disease.
Conclusions: leptospirosis is present in public workers with occupational hazard in Tolima. In addition, this issue should be approached while taking into account the population from other municipalities as well as the
animals associated with its transmission.
|Abstract
= 420 veces | PDF (ESPAÑOL (ESPAÑA))
= 178 veces|

Downloads

Download data is not yet available.

Author Biographies

Blanca L. Guzmán Barragán, National Institute of Health

PhD , MSc, Veterinary Zootechnician, National Institute of Health. Bogota, D.C., Colombia.

Sandra M. Peña Perdomo, Departmental Laboratory of Public Health, Secretariat of Health of Tolima

Bacteriologist, Departmental Laboratory of Public Health, Secretariat of Health of Tolima. Ibagué, Colombia.

Roy F. Flórez Rojas, University of Tolima

Student of Veterinary Medicine and Zootechnics, University of Tolima, Ibagué, Colombia.

Diego F. Amado Gómez, University of Tolima, Secretariat of Health of Tolima

Student of Veterinary Medicine and Zootechnics, University of Tolima. Zoonosis Program Intern, Secretariat of Health of Tolima, Tolima Government, Ibagué, Tolima. Colombia.

Elkin M. Rodríguez Ruíz, University of Tolima, Secretariat of Health of Tolima

Zootechnical Veterinarian, University of Tolima. Zoonosis Program, Secretariat of Health of Tolima, Tolima Government, Ibagué, Tolima. Colombia.

Noel Verjan García, University of Tolima

MSc, PhD., Veterinary Zootechnician, University of Tolima, Ibagué, Colombia.

References

(1). Picardeau M. Diagnosis and epidemiology of leptospirosis. Med Mal Infect 2013; 43 (1): 1-9.

(2). Bharti AR, Nally J, Ricaldi JN, Mathias M, Díaz MM, Lovett MA. Leptospirosis: a zoonotic disease of global importance. Lancet Infect Dis 2003; 3 (12): 757-71.

(3). Hartskeerl RAM, Collares-Pereira, Ellis WA. Emergence, control and re-emerging leptospirosis: dynamics of infection in the changing world. Clin Microbiol Infect 2011; 17(4): 494-501.

(4). Ullmann LS, Langoni H. Interactions between environment, wild animals and human leptospirosis. J Venom Anim Toxins incl Trop Dis 2011; 17 (2): 119-129.

(5). Pappas G, Papadimitriou P, Siozopoulou V, Christou L, Akritidis N. The globalization of leptospirosis: worldwide incidence trends. Int J Infect Dis 2008; 12 (4): 351-357.

(6). Ferro BE, Rodríguez AL, Pérez M, Travi BL. Seroprevalencia de infección por Leptospira en habitantes de barrios periféricos de Cali. Biomédica 2006; 26 (2): 250-257.

(7). Cann KF, Thomas DR, Salmon RL, Wyn-Jones AP, Kay D. Systematic Review. Extreme water-related weather events and waterborne disease. Epidemiol Infect 2013; 141 (4): 671-686.

(8). Arroyave E, Londoño AF, Quintero JC, Agudelo-Flórez P, Arboleda M, Díaz FJ, Rodas JD. Etiología y caracterización epidemiológica del síndrome febril no palúdico en tres municipios del Urabá antioqueño, Colombia. Biomédica 2013; 33 (1) 99-107.

(9). Jaramillo L, Arboleda M, García V, Agudelo-Flórez, P. Coinfección brucelosis-leptospirosis, Urabá, Colombia. Reporte de caso. Infectio 2014; 18 (2): 72-76.

(10). Agudelo PF, Restrepo BNJ, Arboleda MN. Situación de la Leptospirosis en el Urabá antioqueño colombiano: estudio seroepidemiológico y factores de riesgo en población general urbana. Cad de Saúde Pública 2007; 23 (9): 2094-2102.

(11). Góngora A, Parra J, Aponte LH, Gómez LA. Seroprevalencia de Leptospira spp. en grupos de población de Villavicencio, Colombia. Rev Salud Pública 2008; 10 (2): 269-278.

(12). Escobar DF, García WF, Sandoval LA, Tibaquira LE, Grisales H. Epidemiología de la leptospirosis en el departamento del Tolima, Colombia, 2009-2011. Rev Fac Nac Salud Pública 2013; 31 (1): 48-57.

(13). Abela Ridder B, Sikkema R, Hartskeerl RA. Estimating the burden of human leptospirosis. International Journal Antimicrobial Agents 2010; 36 (1): S5-S7.

(14). Costa F, Martínez-Silveira MS, Hagan JE, Hartskeerl RA, Reis MGD, Ko AI. Surveillance for leptospirosis in the Americas, 1996-2005: a review of data from ministries of health. Rev Panam Salud Pública 2012; 32 (3): 169-177.

(15). Bello S. Rodríguez M, Rodríguez F, Paredes A, Realpe M, Mendivelso F. Comportamiento de la vigilancia epidemiológica de la leptospirosis humana en Colombia, 2007-2011. Biomédica 2013; 33 (1): 153-60.

(16). Agudelo-Flórez P, Restrepo M, Lotero MA. Evaluación de la prueba de inmunofluorescencia indirecta para el diagnóstico de leptospirosis humana. Biomédica 2006; 26 (2), 216-23.

(17). World Health Organization. Human leptospirosis: guidance for diagnosis, surveillance and control. Geneva: World Health Organization. 2008.

(18). Champagne F, Hartz Z, Brousselle A, Contandriopoulo|as AP. A apresiacao normativa. En Avaliação: conceitos e métodos. Brousselle A, Champagne F, Contandriopoulos AP, Hartz Z. Avaliação: conceitos e métodos. Fiocruz. Rio Janeiro: Editora Fiocruz, 2011, pp. 29-48.

(19). Donabedian A. Una aproximación a la monitorización de la calidad asistencial (primera parte). Rev Calid Asist 1991; 6 (1): 1-6.

(20). Instituto Nacional de Salud. Protocolo de Vigilancia en Salud Pública para leptospirosis. Bogotá: Instituto Nacional de Salud, 2010.

(21). Bardin, L. Análisis de contenido. Madrid: Ediciones Akal, 1991.

(22). Vasconcelos LM, Cisalpino EO, Vieira MNR, Koury MC. Pesquisa de aglutininas antileptospira em diferentes grupos profissionais na cidade de Londrina, Paraná. Rev Soc Bras Med Trop 1992; 25 (4): 251-255.

(23). López F, Samudio M, de Assis DM, Cabello Á. Seroprevalencia de leptospirosis y factores asociados en trabajadores del servicio de aseo urbano de la Municipalidad de Asunción, Paraguay. Rev Chil Infect 2015; 32 (6): 628-633.

(24). Rodríguez-Parra ME, Bocanegra-Alonso A, Casar-Solares A, Acosta-González RI, Cruz-Hernández NI, Flores-Gutiérrez GH. Epidemiological patterns of Leptospira interrogans among slaughterhouse workers from the Eastern United States-Mexico border region. Afric J Microbiol Res 2012; 6 (7): 1584-1590.

(25). Gonçalves DD, Teles PS, Reis CRD, Lopes FMR, Freire RL, Navarro IT, Freitas JCD. Seroepidemiology and occupational and environmental variables for leptospirosis, brucellosis and toxoplasmosis in slaughterhouse workers in the Paraná State, Brazil. Rev Inst Med Trop Sao Paulo 2006; 48 (3): 135-140.

(26). Pedraza MA, Salamanca EE, Ramírez YR, Ospina MJ, Pulido OM. Seroprevalencia de anticuerpos anti-Leptospira en trabajadores de plantas de sacrificio animal en Boyacá, Colombia. Infectio 2012; 16 (1): 30-36.

(27). Calderón A, Rodríguez V, Máttar S, Arrieta, G. Leptospirosis in pigs, dogs, rodents, humans, and water in an area of the Colombian tropics. Trop Anim Health Prod 2014; 46 (2): 427-432.

(28). Orrego AU, Giraldo G, Ríos BA. Valencia PAP. Leptospirosis en personas de riesgo de quince explotaciones porcinas y de la Central de Sacrificio de Manizales, Colombia. Arch. Med. Vet 2003; 35 (2): 205-213

(29). Niloofa R, Fernando N, de Silva NL, Karunanayake L. Wickramasinghe H, Dikmadugoda N, et al. Diagnosis of leptospirosis: comparison between microscopic agglutination test, IgM-ELISA and IgM rapid immunochromatography test. PloS one 2015; 10 (6): e0129236.

(30). Bajan MD, Ashford DA, Bragg SL, Woods CW, Aye T, Spiegel RA, et al. Evaluation of four commercially available rapid serologic tests for diagnosis of leptospirosis. J Med Microbiol 2003; 41 (2): 803-9.

(31). Romero-Vivas CM, Thiry D, Rodríguez V, Calderón A, Arrieta G, Máttar S, et al. Molecular serovar characterization of Leptospira isolates from animals and water in Colombia. Biomédica 2013; 33 (1): 179-184.

(32). Romero-Vivas CM, Cuello-Pérez M, Agudelo-Flórez P, Thiry D, Levett PN, Falconar AK. Cross-sectional study of Leptospira seroprevalence in humans, rats, mice, and dogs in a main tropical sea-port city. Am J Trop Med Hyg 2013; 88 (1): 178-183.

(33). Agudelo-Flórez P, Arango JC, Merizalde E, Londoño AF, Quiroz VH, Rodas JD. Evidencia serológica de circulación de Leptospira spp en Rattus norvegicus naturalmente expuestos en una zona urbana colombiana. Rev Salud Pública 2010; 12 (6): 990-999.

(34). World Health Organization. Guidelines for drinking water quality. 4th ed. Geneva: World Health Organization, 2011.

(35). Ochoa JE, Sánchez A, Ruíz I. Epidemiología de la leptospirosis en una zona andina de producción pecuaria. Rev Panam Salud Pública 2000; 7 (5): 325-331.

(36). Romero MH, Sánchez JA, Hayek LC. Prevalencia de anticuerpos contra Leptospira en población urbana humana y canina del Departamento del Tolima. Rev Salud Pública 2010; (12 )2: 268-275.

(37). Almenteros C, Arrieta G, Máttar S, Barguil A, Tamayo L, Padilla T, et al. Seroprevalencia de leptospirosis porcina en el departamento de Córdoba. Rev Colomb Cienc Pecu 2004; 17 (2): 141-147.

(38). Giraldo GL, Orrego AU, Betancurth AM. Los roedores como reservorios de Leptospiras en planteles porcinos de la zona central cafetera de Colombia. Arc Med Vet 202; 34 (1): 69-78.

(39). Cristancho-Torres DS, Benítez-Cabrera KA, Góngora-Orjuela A. Conocimientos sobre leptospirosis en estudiantes de veterinaria y seropositividad, Villavicencio, 2011. Orinoquia 2012; 16 (2): 118-124.

(40). López YL, González C, Gallego BN, Moreno AL. Rectoría de la vigilancia en salud pública en el sistema de seguridad social en salud de Colombia: estudio de casos. Biomédica 2009; 29 (4):567-581.

Downloads

Published

2016-05-21

How to Cite

1.
Guzmán Barragán BL, Peña Perdomo SM, Flórez Rojas RF, Amado Gómez DF, Rodríguez Ruíz EM, Verjan García N. Prevalence of Anti-Leptospira spp. Antibodies among People with Occupational Exposure in Tolima Department. Rev. Fac. Nac. Salud Pública [Internet]. 2016 May 21 [cited 2025 Feb. 5];34(2):156-6. Available from: https://revistas.udea.edu.co/index.php/fnsp/article/view/22372

Issue

Vol. 34 No. 2 (2016): Mayo-Agosto

Section

Research

License

The contents of the articles are the responsibility of the authors

The editorial committee has editorial independence from the National School of Public Health "Héctor Abad Gómez" of the University of Antioquia.

The editorial committee is not responsible for aspects related to copying, plagiarism or fraud that may appear in the articles published in it.

When you are going to reproduce and disclose photographs or personal data in printed or digital format, informed consent is required. Therefore, this requirement is required of the author at the time of receipt of the manuscript.

Authors are responsible for obtaining the necessary permissions to reproduce any material protected by reproduction rights.

The authors preserve the moral rights and assign the economic rights that will correspond to the University of Antioquia, to publish it, distribute electronic copies, include them in indexing services, directories or national and international databases in Open Access, under the Creative Commons Attribution license -Not Commercial-Share Equal 4.0 International Commercial (CC BY-NC-SA) which allows others to distribute, remix, retouch, and create from the work in a non-commercial way, as long as the respective credit and license are granted. new creations under the same conditions.

The authors will sign the declaration of transfer of economic rights to the University of Antioquia, after the acceptance of the manuscript.

The editorial committee reserves the right to reject the articles whose authors do not offer satisfactory explanations about the contribution of each author, to meet the criteria of authorship in the submission letter. All authors must meet the four criteria of authorship according to ICMJE: "a) .- That there is a substantial contribution to the conception or design of the article or to the acquisition, analysis or interpretation of the data. b) That they have participated in the design of the research work or in the critical review of its intellectual content. c) .- That has been intervened in the approval of the final version that will be published.d). That they have the capacity to respond to all aspects of the article in order to ensure that issues related to the accuracy or integrity of any part of the work are adequately investigated and resolved. "

Crossref
Scopus
Google Scholar
Europe PMC