Mecanismos de acción de péptidos antibacterianos Ib-M contra Escherichia coli. Un estudio estructural y proteómico. Resultados preliminares
DOI:
https://doi.org/10.17533/udea.iatreia.345509Keywords:
péptidos, antibacterianos, Escherichia coli, relación estructura-actividad, membrana celularAbstract
Los péptidos antimicrobianos (AMPs) son una buena opción terapéutica contra bacterias multidrogoresistentes. Sin embargo, el uso terapéutico de los AMPs se ha obstaculizado, debido a su inestabilidad en ambientes específicos, propensión al clivaje por proteasas o en algunos casos citotoxicidad. Los AMPs exhiben pequeño tamaño, carga neta positiva y anfipaticidad, necesarios para adquirir estructura secundaria, en superficies hidrofóbicas e hidrofílicas, lo que facilita la interacción electrostática con las membranas bacterianas. Los péptidos Ib-M1, Ib-M2 e Ib-M6, que se usarán en este estudio, son análogos sintéticos del péptido Ib-AMP4, nativo de la planta Impatiens balsamina, que fueron obtenidos después de modificar su carga neta e hidrofobicidad mediante la inserción de arginina y triptófano. Estudios de la actividad biológica de Ib-M contra E. coli O157:H7, mostraron actividad inhibitoria menor a 10 µM, sin evidencia de citotoxicidad en células Vero, por lo que se consideran agentes antibacterianos promisorios.
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