In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp.

Authors

  • Laura FLÓREZ-SAMPEDRO Universidad de Antioquia.
  • Wildeman ZAPATA Universidad de Antioquia / Universidad Cooperativa de Colombia https://orcid.org/0000-0002-7351-8738
  • Lina P. Zapata Universidad de Antioquia
  • Amanda Ines MEJÍA Universidad de Antioquia
  • Carolina ARBOLEDA Universidad de Antioquia
  • Maria Teresa RUGELES Universidad de Antioquia https://orcid.org/0000-0002-5517-1195

DOI:

https://doi.org/10.17533/udea.vitae.v23n2a03

Keywords:

Human immunodeficiency virus type 1 (HIV-1), Ganoderma, Lentinus, laccase, antiviral activity, natural products.

Abstract

Background: Natural compounds are a good source for the development of antiretroviral drugs with low cytotoxicity. The laccase enzyme, produced by fungi of the genera Ganoderma sp. and Lentinus sp., inhibits the reverse transcriptase (RT) of the human immunodeficiency virus 1 (HIV-1), in cell-free models in vitro. Aims: In this study we evaluated the anti-HIV-1 activity of the enzymatic crude (EC) enriched with laccase, produced by two native species of fungi of the same genera in an in vitro cell culture model. Methods: The inhibition of viral replication was performed using the U373-MAGI cell line infected with recombinant viruses in the presence/absence of the EC and 48 hpi, the percentage of infected cells was evaluated by flow cytometry for GFP and ELISA for p24. The inhibition of the RT was determined by quantification of early and late products of reverse transcription using quantitative PCR. Results: The ECs from Ganoderma sp. and Lentinus sp. inhibited the replication of HIV-1 between 80 and 90% and decreased the production of early and late transcripts between 55,5%-91,3% and 82,1%-93,6% respectively. The EC from Lentinus sp. had the best selectivity index (SI: 4.77). Conclusions: These results suggest the potential anti-HIV-1 of the EC for the exploration of an alternative therapy against HIV-1 infection.

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Author Biographies

Laura FLÓREZ-SAMPEDRO, Universidad de Antioquia.

Faculty of Medicine, Immunovirology Group, Biology

Wildeman ZAPATA, Universidad de Antioquia / Universidad Cooperativa de Colombia

Faculty of Medicine, Immunovirology Group / Faculty of Medicine, Infettare Group, PhD

Lina P. Zapata, Universidad de Antioquia

Faculty of Medicine, Immunovirology Group, Biology.

Amanda Ines MEJÍA, Universidad de Antioquia

Faculty of Pharmaceutical and Food Sciences, School of Nutrition and Dietetics, Grupo BIOPOLIMER, PhD

Carolina ARBOLEDA, Universidad de Antioquia

Faculty of Pharmaceutical and Food Sciences, School of Nutrition and Dietetics, Grupo BIOPOLIMER, PhD

Maria Teresa RUGELES, Universidad de Antioquia

Faculty of Medicine, Immunovirology Group

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Published

30-09-2016

How to Cite

FLÓREZ-SAMPEDRO, L., ZAPATA, W., Zapata, L. P., MEJÍA, A. I., ARBOLEDA, C., & RUGELES, M. T. (2016). In vitro anti-HIV-1 activity of the enzymatic extract enriched with laccase produced by the fungi Ganoderma sp. and Lentinus sp. Vitae, 23(2), 109–118. https://doi.org/10.17533/udea.vitae.v23n2a03

Issue

Section

Natural Products

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