Wound healing activity of Allium cepa L. bulbs in a second-degree burn wound model in Holtzman rats





Burn, Allium cepa L, Phytochemicals, Burn healing, Silver sulfadiazine, Histology, Onion


Background: The bulb of Allium cepa Linnaeus (onion) is used in traditional medicine as an antidiabetic, antioxidant, antihypertensive, anti-inflammatory, and antihyperlipidemic, among others. The lack of information or little knowledge about the effects of Allium cepa L. on skin lesions, specifically burn wounds, arouses interest in studying its effects on these skin disorders. Objective: This study assessed the wound healing activity of Allium cepa L. on second-degree burns induced in Holtzman rats. Method: Thirty-two albino rats were randomly distributed into four groups of 8 rats each, including the Healthy group, the Control group, the Experimental group (Allium cepa L.), and the Standard group (1% silver sulfadiazine). Burn wounds were induced, and topical treatments were performed daily for 21 days. The reduction of the burned body area (mm2) was determined during the experimental time. Albino rats were sacrificed with an excess of surgical anesthesia to obtain tissue samples for histopathological analysis. Results: Standard and experimental groups significantly reduced burned body area (p<0.01) compared to the control group. Histopathological studies showed hyperemic chorion in the Control group, fibroblasts, and collagen in the Standard group, and dermis composed of a reticular stratum of fibroblasts, collagen, and few blood vessels in the Experimental group. Conclusion: Allium cepa L. revealed wound-healing activity on burns induced in Holtzman rats and reduced the damage produced by burns.

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Author Biography

Carmen Marín-Tello, Universidad Nacional de Trujillo

Department of Pharmacology. Universidad Nacional de Trujillo. 


Lukiswanto BS, Miranti A, Sudjarwo SA, Primarizky H, Yuniarti WM. Evaluation of wound healing potential of pomegranate (Punica granatum) whole fruit extract on skin burn wound in rats (Rattus norvegicus). J Adv Vet Anim Res. 2019; 6(2):202-207. DOI: http://doi.org/10.5455/javar.2019.f333

Schencke C, Vasconcellos A, Salvo J, Veuthey C, Del Sol M. Efecto cicatrizante de la miel de ulmo (Eucryphia cordifolia) suplementada con ácido ascórbico como tratamiento en quemaduras. Int. J. Morphol. 2015; 33(1):137-143. DOI: http://dx.doi.org/10.4067/S0717-95022015000100022

World Health Organization. Burns [Internet], https://www.who.int/es/news-room/fact-sheets/detail/burns 2020 (accessed 06 December 2020).

ANDINA. Agencia peruana de noticias. Cada año se reportan en Perú unos 15,000 casos de niños quemados. [Internet], https://andina.pe/agencia/noticia-cada-ano-se-reportan-peru-unos-15000-casos-ninos-quemados-757545.aspx 07/11/2019 (accessed 08 December 2020)

Akhoondinasab MR, Akhoodinasab M, Saberi M. Comparison of healing effect of Aloe vera extract and silver sulfadiazine in burn injuries in experimental rat model. World J Plast Surg 2014; 3(1):29-34. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4236981/

Lozada D. Recuperación de la integridad de la piel en ratas inducidas a quemaduras tratadas con extracto hidroalcohólico de Spondias mombin (Ulcos). 2020. [Grade Work]. [Lima, Peru]: Universidad María Auxiliadora. 2020. 88 p. Available from: http://repositorio.uma.edu.pe/handle/UMA/226

Vaghardoost R, Ghavami y, Sobouti B. The effect of Mentha pulegium on healing of burn wound injuries in rat. World J Plast Sur. 2019; 8(1):43-50. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409140/

Cueto T. Situación fitosanitaria de la cebolla roja (Allium cepa), en Barranca. [Grade Work]. [Lima, Peru]: Universidad Nacional de La Molina. 2017.48 p. Available from: http://repositorio.lamolina.edu.pe/handle/UNALM/2958

Bystricka J, Musilova J, Vollmannova A, Timoracka M, Kalcova P. Bioactive components of onion (Allium cepa L.)- A review. Acta Alimentaria. 2013; 42(1):11-22. DOI: https://doi.org/10.1556/aalim.42.2013.1.2

National Research Council (US) Committee for the Update of the Guide for the Care and Use of Laboratory Animals. Guide for the Care and Use of Laboratory Animals. [Internet] 8th ed. Washington DC. National Academies Press (US). 2011. [Cited 2020 December 16].220p. Available from: https://www.ncbi.nlm.nih.gov/books/NBK54050/

Lock O. Investigación fitoquímica. Métodos en el estudio de productos naturales. 3ª ed., Fondo Editorial Pontificia Universidad Católica del Perú, Lima; 2013. 287p.

Gennaro A. Remington: Farmacia.Tomo.1. 20 ed., Médica Panamericana S.A, Buenos Aires; 2003.3894p.

Hawk CT, Leary S, Morris T. Formulary for laboratory animals. [Internet]. Iowa: Backwell Publishing; 2005 [cited 2021 January 27]. 196 p. Available from: https://www.upstate.edu/iacuc/pdf/Formulary_for_Lab_Animals_3rd_ed.pdf

Corzo Martínez M, Corzo N, Villamiel M. Biological properties of onion and garlic. Trends Food Sci. Technol.2007; 18(12):609-625. DOI: https://doi.org/10.1016/j.tifs.2007.07.011

Rodríguez Galdón B, Rodríguez Rodríguez E, Díaz Romero C. Flavonoids in onion cultivars (Allium cepa L.). J Food Sci. 2008;3(8):C599-605. DOI: https://doi.org/10.1111/j.1750-3841.2008.00903.x

Dalhat M, Adefolake F, Musa M. Nutritional composition and phytochemical analysis of aqueous extract of Allium cepa (onion) and Allium sativum (garlic). Asian Food Science Journal. 2018; 3(4): 1-9. DOI: https://doi.org/10.9734/AFSJ/2018/43165

Boukeria S, Kadi K, Kalleb R, Benbott A, Bendjedou D, Yahia A. Phytochemical and physicochemical characterization of Allium sativum L. and Allium cepa L. Essential oils. J. Mater. Environ. Sci. 2016 ; 7(7):2240-2257. Available from: https://www.jmaterenvironsci.com/Document/vol7/vol7_N7/254-JMES-2066-Boukeria.pdf

Farag M, Ali S, Hodaya R, El-Seedi H, Sultani H, Laub A, et al. Phytochemical orofiles and antimicrobial activities of Allium cepa Red cv. and A. sativum subjected to different drying methods: A comparative MS-Based metabolomics. Molecules.2017; 22(5):761. DOI: https://doi.org/10.3390/molecules22050761

Shenoy C, Patil MB, Kumar R, Patil S. Preliminary phytochemical investigation and wound healing activity of Allium cepa (Liliaceae). Int J Pharm Pharm Sci. 2009;1(2):167-175. Available from: https://innovareacademics.in/journal/ijpps/Vol1Issue2/208.pdf

Durmus A, Yaman M, Can H. Effects of extractum cepae, heparin, allantoin gel and silver sulfadiazine on burn wound healing: an experimental study in a rat model. Vet Med (Praha). 2012;57(6):287-292. DOI: https://doi.org/10.17221/6015-VETMED

Kimura Y, Sumiyoshi M, Kawahira K, Sakanaka M. Effect of ginseng saponins isolated from Red Ginseng roots on burn wound healing in mice. Br J Pharmacol. 2000; 148(6):860-870. DOI: https://doi.org/10.1038/sj.bjp.0706794

Upadhyay R. Nutraceutical, pharmaceutical and therapeutic uses of Allium cepa: A review. Int. J. Green Pharm. 2016 (Suppl). 10(1): S46-S64. DOI: http://dx.doi.org/10.22377/ijgp.v10i1.612

Ben-Kedir S, Bardaa S, Chabchoub N, Moalla D, Sahnoun Z, Rebai T. The healing effect of Pistacia lentiscus fruit oil on laser burn. Pharm Biol. 2017; 55(1):1407-1414. DOI: https://doi.org/10.1080/13880209.2016.1233569

Egri, Ö, Erdemir N, Gevrek F. Effect of H. perforatum oil containing membranes on the second degree burn wounds in rats. Mater Today Commun. 2020; Vol. 24, 100954. DOI: https://doi.org/10.1016/j.mtcomm.2020.100954

Alca, E, Del Solar M, Bravo F. Efecto de la xantopterina en la cicatrización de heridas por quemaduras en ratas. Folia Dermatol. 2009; 20(1):13-17. Available from: https://sisbib.unmsm.edu.pe/BVRevistas/folia/vol20_n1/pdf/a03v20n1.pdf

Bayir Y, Un H, Ugan R, Akpinar E, Cadirci E, Calik I, et al. The effects of Beeswax, Olive oil and Butter impregnated bandage on burn wound healing. Burns. 2019; 45(6):1410-1417. DOI: https://doi.org/10.1016/j.burns.2018.03.004

Tsala D, Joseph M, Simplice T, Nga N, Jacques E, David M. Effect of methanol extract of Allium cepa Linn. on incisional wound healing in alloxan-induced diabetic mice. Appl Med Res. 2015; 1(3):90-93. DOI: https://dx.doi.org/10.5455/amr.20150427021827

Tuhin R, Begum M, Rahman M, Karim R, Begum T, Ahmed S, et al. Wound healing effect of Euphorbia hirta Linn. (Euphorbiaceae) in alloxan induced diabetic rats. BMC Compl Alternative Med. 2017; 17(1), 423. DOI: https://doi.org/10.1186/s12906-017-1930-x

Lanzotti V. The analysis of onion and garlic. J Chromatogr A. 1112 (1-2): 3- 22. DOI: https://doi.org/10.1016/j.chroma.2005.12.016

Jaiswal N, Kumar D, Rizvi S. Red onion extract (Allium cepa L.) supplementation improves redox balance in oxidatively stressed rats. Food Sci. Hum. Wellness. 2013; 2(2):99-104. DOI: https://doi.org/10.1016/j.fshw.2013.05.003

Heidari M, Bahramsoltani R, Abdolghaffari A, Rahimi R, Esfandyari M, Baeeri M, et al. Efficacy of topical application of standardized extract of Tragopogon

graminifolius in the healing process of experimental burn wounds. J Tradit Complement Med. 2019; 9(1):54-59. DOI: https://doi.org/10.1016/j.jtcme.2018.02.002

MacKay D, Miller A. Nutritional support for wound healing. Altern Med Rev. 2003; 8(4):359-377. Available from: https://pubmed.ncbi.nlm.nih.gov/14653765/

Fain O. Vitamin C deficiency. Revue Med Interne. 2004; 25:872-880. DOI: https://doi.org/10.1016/j.revmed.2004.03.009

Pikula M, Zebrowska ML, Poblocka-Olech L, Krauze-Baranowska M, Sznitowska M, Trzonkowski P. Effect of enoxaparin and onion extract on human skin fibroblast cell line- therapeutic implications for the treatment of keloids. Pharm Biol. 2014; 52(2):262-267. DOI: https://doi.org/10.3109/13880209.2013.826246

Zurada J, Kriegel D, Davis I. Topical treatments for hypertrophic scars. J Am Acad Dermatol 2006; 55(6):1024-1031. DOI: https://doi.org/10.1016/j.jaad.2006.03.022




How to Cite

Guevara-Vásquez, A. M., & Marín-Tello, C. (2021). Wound healing activity of Allium cepa L. bulbs in a second-degree burn wound model in Holtzman rats. Vitae, 28(3). https://doi.org/10.17533/udea.vitae.v28n3a345737



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