Desarrollo de un método de análisis para la determinación de mefloquina en sangre humana secada sobre papel de filtro por cromatografía liquida de alta resolución con detección ultravioleta
DOI:
https://doi.org/10.17533/udea.vitae.454Keywords:
Mefloquina, validaciòn, estudios de recuperaciòn, Mefloquine, validation, recovery studiesAbstract
Se valida el método de análisis para determinar mefloquina en sangre humana secada sobre papel de filtro Wathman No. 3 por HPLC con detección ultravioleta. Se hace extracción líquido-líquido y la separación se realiza con una columna C18 por elución isocrática; la fase móvil utilizada fue acetonitrilo : fosfato de potasio monobasico (40:60) y el analito fue monitoreado a 222 nm. Los límites de detección y cuantificación son de 97,5 ng / mL y 136,2 ng / mL respectivamente. La separación cromatográfica del analito demora 9 minutos y la cuantificación se hace por el método del estándar externo. Este método es relativamente simple, rápido y sensible y puede utilizarse para el monitoreo de estudios clínicos y farmacocinéticos.Downloads
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