COMPARISON OF RESAZURIN AND MTT METHODS ON STUDIES OF CITOTOXICITY IN HUMAN TUMOR CELL LINES

Authors

  • Linamaría ESCOBAR M. Grupo de Farmacogenética del Cáncer. Departamento de Farmacia. Facultad de Ciencias. Universidad Nacional de Colombia. Sede Bogotá.
  • Augusto RIVERA Departamento de Química, Facultad de Ciencias, Universidad Nacional de Colombia. Sede Bogotá
  • Fabio A. ARISTIZÁBAL G. Grupo de Farmacogenética del Cáncer. Departamento de Farmacia. Facultad de Ciencias. Universidad Nacional de Colombia. Sede Bogotá.

DOI:

https://doi.org/10.17533/udea.vitae.4977

Keywords:

Doxorubicin, MTT, resazurine, cellular viability.

Abstract

A study was carried comparing the resazurina and MTT methods as indicators of indirect measure of
cellular survival applied to valuated cytotoxic activity in order to assess the suitability of each of the two
detection systems. The comparison was made through the valuation of cytotoxicity on three cell lines
derived from human tumors: HEp-2, HT-29 and HeLa, evaluating three synthetic compounds during
a treatment period of 48 hours using as a reference activity Doxorubicin HCl. The results obtained by
the two methods showed a similar performance under the conditions tested in cell lines
|Abstract
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Published

26-03-2010

How to Cite

ESCOBAR M., L., RIVERA, A., & ARISTIZÁBAL G., F. A. (2010). COMPARISON OF RESAZURIN AND MTT METHODS ON STUDIES OF CITOTOXICITY IN HUMAN TUMOR CELL LINES. Vitae, 17(1), 67–74. https://doi.org/10.17533/udea.vitae.4977

Issue

Section

Pharmacology and Toxicology