CELLULAR SUSPENSIONS ESTABLISHMENT OF Bixa orellana L., PHENOLIC COMPOUNDS PRODUCERS WITH POTENTIAL ANTIOPHIDIC ACTIVITY.
DOI:
https://doi.org/10.17533/udea.vitae.768Keywords:
cells suspensions, Bixa orellana L, phenolics compoundsAbstract
A protocol for production and massification of achiote cells in suspension from friable calli of leaf tissues, has been
developed as a strategy to obtain antiophidics metabolites, especially phenolics compounds. Concentration effects
of, inoculum, glucose, phosphorus and nitrogen, on cellular growth kinetics were evaluated in ½ MS+2,4-D
(5 ppm)+BAP (1 ppm) medium, stored at 25º C, darkness and 140 rpm, using a randomized factorial design with
4 factors, two levels and evaluation at 20 and 40 days of establishment. Treatment with highest biomass production
of achiote cells in suspension, had an initial biomass concentration of 4g/l, glucose 20g/l, phosphorus 0,13g/l and
nitrogen 2,52 g/l. Kinetics growth of achiote cells in suspension in culture conditions, presented a well defined
exponential phase of 25 days, since then a stationary phase until the end of the evaluation (40 days). Total phenols
contents among material obtained in-vitro and vegetable material obtained ex vitro were compared as a
valid criteria to justify later works of in -vitro metabolic production in this vegetable specie.
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