CELLULAR SUSPENSIONS ESTABLISHMENT OF Bixa orellana L., PHENOLIC COMPOUNDS PRODUCERS WITH POTENTIAL ANTIOPHIDIC ACTIVITY.

Authors

  • Silvia L. JIMÉNEZ R.
  • Hader I. CASTAÑO P.
  • Juan Carlos QUINTANA
  • Gelmy L. CIRO G.
  • Lida QUINCHÍA
  • Sebastián ESTRADA

DOI:

https://doi.org/10.17533/udea.vitae.768

Keywords:

cells suspensions, Bixa orellana L, phenolics compounds

Abstract

A protocol for production and massification of achiote cells in suspension from friable calli of leaf tissues, has been

developed as a strategy to obtain antiophidics metabolites, especially phenolics compounds. Concentration effects

of, inoculum, glucose, phosphorus and nitrogen, on cellular growth kinetics were evaluated in ½ MS+2,4-D

(5 ppm)+BAP (1 ppm) medium, stored at 25º C, darkness and 140 rpm, using a randomized factorial design with

4 factors, two levels and evaluation at 20 and 40 days of establishment. Treatment with highest biomass production

of achiote cells in suspension, had an initial biomass concentration of 4g/l, glucose 20g/l, phosphorus 0,13g/l and

nitrogen 2,52 g/l. Kinetics growth of achiote cells in suspension in culture conditions, presented a well defined

exponential phase of 25 days, since then a stationary phase until the end of the evaluation (40 days). Total phenols

contents among material obtained in-vitro and vegetable material obtained ex vitro were compared as a

valid criteria to justify later works of in -vitro metabolic production in this vegetable specie.

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Published

16-02-2009

How to Cite

JIMÉNEZ R., S. L., CASTAÑO P., H. I., QUINTANA, J. C., CIRO G., G. L., QUINCHÍA, L., & ESTRADA, S. (2009). CELLULAR SUSPENSIONS ESTABLISHMENT OF <I>Bixa orellana</I> L., PHENOLIC COMPOUNDS PRODUCERS WITH POTENTIAL ANTIOPHIDIC ACTIVITY. Vitae, 15(1). https://doi.org/10.17533/udea.vitae.768

Issue

Section

Biotechnology

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