Identification of Genotoxic Damage Caused by Occupational Exposure to BTEX in Footwear Industry Workers in Bucaramanga Between 2019 - 2020 Using the Conventional Micronuclei Technique
Keywords:
Benzene Derivatives, Occupational Exposure, Micronucleus Tests, Abdominal Neoplasms, Genetic PhenomenaAbstract
Introduction: Occupational exposure to BTEX can cause genetic and epigenetic damage, potentially leading to cancer.
Objective: To determine the association between occupational exposure to BTEX and genetic alterations in exposed workers.
Methodology: This project is part of a larger project endorsed by Minciencias (Code 126780763345). It is an experimental cross-sectional study. After obtaining informed consent, peripheral blood samples were collected, and the Fenech et al. micronuclei technique was applied to 2x500 binucleated cells.
Results: To date, sociodemographic information from 159 workers has been analyzed. Of the total individuals, 70.8% are female, and 29.2% are male. 46.1% are young adults (18-39 years old), and 49.2% are adults (40-64 years old). 54.3% have completed only high school education, 36.2% have completed primary education, and only 58% are professionals. The majority belong to socioeconomic stratum 2 (62.6%). Currently, 96 samples have been analyzed using the conventional micronuclei technique, revealing an average of 7.1 micronuclei per 500 binucleated cells.
Conclusions: This study demonstrates an association between occupational exposure to BTEX and genetic alterations in footwear industry workers. The results suggest the need for preventive measures to reduce BTEX exposure and protect the health of workers.
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References
Fenech M, Chang W, Kirsch-Volders M, Holland N, Bonassi S, Zeiger E, et al. HUMAN Project: Detailed description of the scoring criteria for the cytokinesisblock micronucleus assay using isolated human lymphocyte cultures. Mutat Res
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Fenech M. Cytokinesis-block micronucleus cytome assay. Nat Protoc [Internet]. 2007;2(5):1084-1104. https://doi.org/10.1038/nprot.2007.77
Perry P, Wolff S. New Giemsa method for the differential staining of sister chromatids. Nature [Internet]. 1974;251(5471):156-8. https://doi.org/10.1038/251156a0
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