Comparison of the Effectiveness of Leaf Extracts of Anredera cordifolia, Psidium guajava and Pogostemon cablin on Inhibitory Power Over Escherichia coli Bacteria


  • Budianto Budianto Institute Science and Technology Al-Kamal, Jakarta, Indonesia
  • Alif Gita Arum sari
  • Nurul Inayah
  • F. Fatmaningrum
  • Anik Suparmi



Anti-bacterial, Antimicrobial, agar diffusion, Anredera cordifolia, Psidium guajava, Pogostemon cablin, TLC, Escherichia coli


Background: the current research studies why it is effective using Anredera cordifolia, Psidium guajava, and Pogostemon cablin by the local community as a traditional medicine for diarrhea treatment caused by Escherichia colibacteria. Objectives: We compared the inhibitor effectiveness of three leaf extracts against Escherichia coli; we also identified the anti-bacterial substances contained in leaf extracts. Methods: We determined the bacterial test activity using the "agar diffusion" method and the thin layer chromatography (TLC) as qualitative analysis for determining the anti-bacterial substances contained in the extract. Results: The Pogostemon cablin leaf extract contained terpenoids, phenolic, and flavonoids compound as bacterial inhibitors, and the comparison showed that Pogostemon cablin leaf extract had the greatest bacterial inhibition power. Conclusion: The antibiotic substances found in the leaf extracts of Anredera cordifolia, Psidium guajava, and Pogostemon cablin can be used as traditional medicine. The breakthrough was evidenced by the ability to inhibit Escherichia coli bacteria. This research shows that traditional medicine has ancient knowledge used by this paper

= 465 veces | PDF
= 305 veces|


Download data is not yet available.


M. Farthing et al., “Acute Diarrhea in Adults and Children: A Global Perspective,” ,Journal of Clinical Gastroenterology: 2013; 47.1: 12-20. DOI:

C. L. F. Walker et al., “Global burden of childhood pneumonia and diarrhoea,” Lancet, 2013; 381.9875: 1405–1416. DOI:

K. Allison, “Book Review: WHO global atlas of traditional, complementary and alternative medicine,” J. R. Soc. Promot. Health, Kobe; 2005. 103p.

B. Biswas, K. Rogers, F. McLaughlin, D. Daniels, and A. Yadav, “Antimicrobial activities of leaf extracts of guava (psidium guajava L.) on two gram-negative and gram-positive bacteria,” Int. J. Microbiol., 2013; 746165: 7 pages DOI:

J. B. Harborne and C. A. Williams, “Advances in flavonoid research since 1992,” Phytochemistry. 2000; 55.6: 481-504. DOI:

J. H. Doughari, “Antimicrobial Activity of Tamarindus indica Linn,” Trop. J. Pharm. Res., 2006; 5.2: 597-603. DOI: Available at

P. S. Stewart and J. W. Costerton, “Antibiotic resistance of bacteria in biofilms,” Lancet. 2001; 358.9276: 135-138. DOI:

T. G. Taruscio, D. L. Barney, and J. Exon, “Content and Profile of Flavanoid and Phenolic Acid Compounds in Conjunction with the Antioxidant Capacity for a Variety of Northwest Vaccinium Berries,” J. Agric. Food Chem., 2004; 52. 10:3169–3176. DOI:

S. M. Astuti, M. Sakinah A.M, R. Andayani B.M, and A. Risch, “Determination of Saponin Compound from Anredera cordifolia (Ten) Steenis Plant (Binahong) to Potential Treatment for Several Diseases,” J. Agric. Sci., 2011; 3.4: 224-232. DOI:

N. P. E. Leliqia, E. Y. Sukandar, and I. Fidrianny, “Antibacterial activities of Anredera Cordifolia (Ten.) V. Steenis leaves extracts and fractions,” Asian J. Pharm. Clin. Res., 2017;3 .4: 224-232. DOI:

V. Wijaya, E. S. Maharani, H. A. Gunawan, and R. Puspitawati, “The efficacy of an infusion of binahong leaves (Anredera cordifolia (ten.) steenis) against wild strain black-pigmented bacteria,” Int. J. Appl. Pharm., 2017; 9.2: 28-31. DOI:

E. S. Maharani, R. Puspitawati, and H. A. Gunawan, “Antibacterial effect of binahong (Anredera cordifolia (Ten.) Steenis) leaf infusion against black pigmented bacteria,” in Journal of Physics: Conference Series, 2018; 1073. 03. DOI: 6596/1073/3/032013

M. D. Mahfuzul Hoque, M. L. Bari, Y. Inatsu, V. K. Juneja, and S. Kawamoto, “Antibacterial activity of guava (Psidium guajava L.) and neem (Azadirachta indica A. Juss.) extracts against foodborne pathogens and spoilage bacteria,” Foodborne Pathog. Dis., 2007; 4.4: 481-488. DOI:

A. Pachanawan, P. Phumkhachorn, and P. Rattanachaikunsopon, “Potential of Psidium guajava Supplemented Fish Diets in Controlling Aeromonas hydrophila Infection in Tilapia (Oreochromis niloticus),” J. Biosci. Bioeng., 2008; 106. 5: 419-424. DOI:

L. F. Hu et al., “GC-MS fingerprint of Pogostemon cablin in China,” J. Pharm. Biomed. Anal., 2006; 42.2: 200-206. DOI: Available at

P. Chakrapani et al., “Comparitive studies on antibacterial activity of Patchouli [Pogostemon cablin (Blanco) Benth] and Geranium (Pelargonium graveolens) aromatic medicinal plants,” African J. Biotechnol., 2014; 13.23: 2379-2384. DOI:

F. Peng, F. Wan, L. Xiong, C. Peng, M. Dai, and J. P. Chen, “In vitro and in vivo antibacterial activity of Pogostone,” Chin. Med. J. (Engl)., 2014; 127. 23 : 4001-4005 doi:

S. Widyaningrum and S. Nurjanah, “NILAM TERHADAP BAKTERI ESCHERICIA COLI DAN PSEUDOMONAS AERUGINOSA Antibacterial Activity ,”Gontor Agriculture. 2020; 6. 3:403–412. DOI:

Y. Deguchi and K. Miyazaki, “Anti-hyperglycemic and anti-hyperlipidemic effects of guava leaf extract,” Nutrition and Metabolism. 2010; 7, 9. DOI:

Schnitzler P. Psidium Guajava (Guava): A Plant of Multipurpose Medicinal Applications. Med Aromat Plants. 2012; 01.04. DOI: :

S. Munda, S. Dutta, S. K. Pandey, N. Sarma, and M. Lal, “Antimicrobial Activity of Essential Oils of Medicinal and Aromatic Plants of the North East India: A Biodiversity Hot Spot,” J. Essent. Oil-Bearing Plants, 2019; 22.1: 105- 119. DOI:

D. L. Valle, J. I. Andrade, J. J. M. Puzon, E. C. Cabrera, and W. L. Rivera, “Antibacterial activities of ethanol extracts of Philippine medicinal plants against multidrug-resistant bacteria,” Asian Pac. J. Trop. Biomed., 2015;5.7 :532-540 DOI:

Zuhaira S, Nizam NM, Ridzuan P. The Efficacy of Psidium guajava Linn Leaf Extractsfrom Selangor Region Against Gram-Positive and Gram-Negative Bacteria. Folia Medica Indones. 2018; 54.4:294. doi:

S. Ramli, S. Radu, K. Shaari, and Y. Rukayadi, “Antibacterial activity of ethanolic extract of syzygium polyanthum L. (Salam) leaves against foodborne pathogens and application as food sanitizer,” Biomed Res. Int., 2017; vol.2017. ID 9024246: 13 pages, 2017. DOI:

Badan POM RI, “Peraturan Kepala Badan Pengawas Obat Dan Makanan Republik Indonesia Tahun 2011 Tentang Metode Analisis Kosmetika, first ed. BPOM, jakarta,” pp. 1–92, 2011. 1–92p

Kemendikbud, Buku Informasi Melaksanakan Analisa Secara Kromatografi Konvensional Mengikuti Prosedur. jakarta; 2018. 1-80p

F. Geiss, Fundamentals of thin layer chromatography. United States: Huethig Publishing,Mamaroneck, NY, 1987. Available at

Charalampos Proestos, Michael Komaitis, Application of microwave-assisted extraction to the fast extraction of plant phenolic compounds,LWT - Food Science and Technology, 2008; 41.4: 652-659. DOI:

Indarto I, Narulita W, Anggoro BS, Novitasari A. Aktivitas Antibakteri Ekstrak Daun Binahong Terhadap Propionibacterium Acnes. Biosf J Tadris Biol. 2019;10.1: 67-78. doi:

M. R. V. Fernandes, A. L. T. Dias, R. R. Carvalho, C. R. F. Souza, and W. P. Oliveira, “Antioxidant and antimicrobial activities of Psidium guajava L. spray dried extracts,” Ind. Crops Prod., 2014. 60: 39-44. DOI:

S. S. Dakappa, R. Adhikari, S. S. Timilsina, and S. Sajjekhan, “A REVIEW ON THE MEDICINAL PLANT PSIDIUM GUAJAVA LINN. (MYRTACEAE),” J. Drug Deliv. Ther., 2013. 3.2. DOI: Available from:




How to Cite

Budianto, B., Arum sari, A. G. ., Inayah, N., Fatmaningrum, F., & Suparmi, A. (2021). Comparison of the Effectiveness of Leaf Extracts of Anredera cordifolia, Psidium guajava and Pogostemon cablin on Inhibitory Power Over Escherichia coli Bacteria. Vitae, 28(3).



Natural Products

Similar Articles

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 > >> 

You may also start an advanced similarity search for this article.