DEVELOPMENT OF DIRECT BIOAUTOGRAPHY AS REFERENCE METHOD FOR TESTING ANTIMICROBIAL ACTIVITY OF GENTAMICIN AGAINST Escherichia coli

Authors

  • Jhonny COLORADO R.
  • Elkin GALEANO J.
  • Alejandro MARTÍNEZ M.

Keywords:

Direct bioautography, gentamicin, Escherichia coli.

Abstract


Bioautography is a fast and simple technique which combines the advantages of thin layer chromatography separation and antimicrobial activity detection. This technique displays directly antimicrobial fraction spots from a mixture. Efficacy determination of the technique improves the isolation of antimicrobial substances from complex mixtures. This article describes how bioautography is useful to quantify antimicrobial activity of amino glycoside gentamicin, against pathogen Escherichia coli (E. coli). Quantitative analysis is verified measuring diffusion halos diameters and applying different concentrations of gentamicin on tlc plates. Volume effect is measured on variability of results. Differences between diffusion halos and microbial inhibition are analyzed too. Results show how antibiotic diffusion on tlc plates is a determining factor, and there is a concentration range where strong interactions between measured variables led to establish a method to be validated for detecting and quantifying antimicrobial substances from complex mixtures.

|Abstract
= 32 veces | PDF (ESPAÑOL (ESPAÑA))
= 15 veces|

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Published

2009-02-04

How to Cite

COLORADO R., J., GALEANO J., E., & MARTÍNEZ M., A. (2009). DEVELOPMENT OF DIRECT BIOAUTOGRAPHY AS REFERENCE METHOD FOR TESTING ANTIMICROBIAL ACTIVITY OF GENTAMICIN AGAINST <I>Escherichia coli</I>. Vitae, 14(1). Retrieved from https://revistas.udea.edu.co/index.php/vitae/article/view/595

Issue

Section

Natural Products

Most read articles by the same author(s)

1 2 > >> 

Similar Articles

1 2 3 4 5 > >> 

You may also start an advanced similarity search for this article.