Participation of NO in the vasodilatory action of isoespintanol

Keywords: Isoespintanol, thoracic aorta, nitric oxide (NO).

Abstract

Background: accumulating evidence suggests that natural compounds and specifically monoterpenes exert a vasodilator action. Objetive: to investigate the vascular effects of isoespintanol (2-isopropil-3,6- dimetoxi-5-metilfenol, ISO) monoterpene isolated from the leaves of Oxandra cf xylopioides. Methods: thoracic aortic rings isolated from Wistar rats were contracted with KCl 80 mM and then relaxed by exposure to Ca2+-free solution in absence and in presence of ISO 0.6 mg/mL. The force/tissue ratio (F/W) and the time to obtain 50% of relaxation (T-50) were used to assess the maximal contractile response and the relaxation, respectively. To examine the participation of NO additional experiments were performed under inhibition of nitric oxide synthase with L-NAME (L-NG-Nitroarginine methyl ester). Results: ISO significantly decreased the F/W ratio (257 ± 19 vs. 360 ± 18) and did not change T-50. In presence of L-NAME the effects of ISO on contractile response was abolished. Conclusions: these results demonstrate that ISO exerts a vasodilator effect through NO- dependent pathways and suggest that an inhibition of calcium influx could be the involved mechanism.
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Author Biographies

Gustavo J. RINALDI, Universidad Nacional de la Plata
Facultad de Ciencias Exactas
Benjamín ROJANO, Universidad Nacional de Colombia Sede Medellín
Facultad de Ciencias, Laboratorio de Ciencias de los Alimentos
Guillermo SCHINELLA, Universidad Nacional de La Plata
Facultad de Ciencias Médicas
Susana M. MOSCA, Universidad Nacional de La Plata
Centro de Investigaciones Cardiovasculares "Dr Horacio E. Cingolani", CCT-CONICET
Published
2019-11-07
Section
Pharmacology and Toxicology