FRUCTOSE SYRUP OBTENTION FROM ENZYMATIC HYDROLYSED OF YAM STARCH (Dioscorea alata and Dioscorea rotundata)

  • Jairo G. SALCEDO M. Universidad de Sucre. Departamento de Ingeniería Agroindustrial
  • Everaldo J. MONTES M. Universidad de Córdoba. Departamento de Ingeniería de Alimentos
  • José E. ZAPATA M. Universidad de Antioquía. Departamento de Ingeniería de Alimentos
  • Didier A. MÁRQUEZ H. Universidad de Sucre. Departamento de Biología.
  • Miguel DIAZ A. Universidad de Sucre. Departamento de Biología.
Keywords: alpha-amylase, hydrolysis, substrate, yam, yield.

Abstract

This investigation evaluated the yield of fructose syrup production from extracted starch of two yam species ( Dioscorea alata , var. Diamond and Dioscorea rotundata , var. Espino). The experiment was conducted using an at random experimental design of multiple categorical factor analysis with two factors: a substrate concentration factor at five levels: 10, 20, 30, 40, 50% p/v and the yam species factor at two levels ( Dioscorea alata , var Diamond and Dioscorea rotundata , var Espino). To obtain fructose, initially glucose syrups were prepared by enzymatic hydrolysis of yam starch using the enzymes of Novozymes®,alpha-amylase Termamyl 120L (liquefaction stage) and AMG 300L (saccharification stage). At this stage, dextrose maximum equivalents (ED) obtained were 98.28 and 96.84 for a substrate concentration of 30%w/v. In a later stage isomerisation from glucose syrups to fructose syrups using the immobilized enzyme Sweetzyme of Novozymes® was carried out. The best results were achieved with substrate concentrations of 30% p/v for both yam species, which showed fructose concentrations of 220.57 and 210.36 g/L with conversions of 71.46% and 67.28% in Espino and Diamond yams respectively. No statistically significant differences were observed between the yam species ( D. alata var. Diamante and D. rotundata var. Espino) at a confidence level of 5%, regarding the production of fructose syrup.
|Abstract
= 21 veces | PDF (ESPAÑOL (ESPAÑA))
= 148 veces|

Downloads

Download data is not yet available.
Published
2010-11-08
How to Cite
SALCEDO M., J. G., MONTES M., E. J., ZAPATA M., J. E., MÁRQUEZ H., D. A., & DIAZ A., M. (2010). FRUCTOSE SYRUP OBTENTION FROM ENZYMATIC HYDROLYSED OF YAM STARCH (<I>Dioscorea alata</I> and <I>Dioscorea rotundata</I&gt;). Vitae, 17(3), 243-251. Retrieved from https://revistas.udea.edu.co/index.php/vitae/article/view/7427
Section
Foods: Science, Engineering and Technology