Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis

  • Johanny Aguillón Osma Universidad del Quindío
  • María Elena Maldonado Universidad de Antioquia
  • Nelsy Loango Chamorro Universidad del Quindío
  • Sandra Sulay Arango Varela Instituto Tecnológico Metropolitano de Medellín
  • Patricia Landázuri Universidad del Quindío
Palabras clave: Passiflora edulis, antioxidante, antiproliferativo, citotóxico

Resumen

Background: Extracts from a variety of fruit trees have been used for therapeutic applications for preventing oxidative stress associated to chronic diseases. Objective: To investigate the antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits of Passiflora edulis. Materials and methods: A preliminary phytochemical screening was performed; antioxidant activity was evaluated through DPPH assay, the scavenging activity for hydroxyl radical, antihemolytic activity and total phenolic content; cytotoxic and antiproliferative activities were evaluated by MTT and sulforhodamine B assays respectively in colon adenocarcinoma SW480 cells and their metastatic-derived SW620 cells. Results: Phytochemical analyses revealed presence of tannins, flavonoids and cardiotonic glycosides. Ethanolic extract from leaves showed the best antioxidant activity (EC50 = 0.096 mg/ml) in the DPPH assay and the juice (EC50 = 0.022 mg/ml) for the Hydroxyl free radical-scavenging activity. All extracts inhibited more than 98% the hemolysis induced by H2 O2 . The aqueous extract from leaves showed the highest cytotoxic activity against SW480 and SW620 cells. Conclusions: Findings from this study suggest that P. edulis is a potential source of phytochemical compounds with antioxidant and antiproliferative properties.
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Biografía del autor/a

Johanny Aguillón Osma, Universidad del Quindío
Grupo de Estudio en Enfermedades Cardiovasculares y Metabólicas (GECAVYME). Facultad de Ciencias de la Salud, Universidad del Quindío. Armenia-Colombia.
María Elena Maldonado, Universidad de Antioquia
Grupo de Investigación Impacto de los Componentes Alimentarios en la Salud (ICAS). Escuela de Nutrición y Dietética, Universidad de Antioquia. Medellín-Colombia.
Nelsy Loango Chamorro, Universidad del Quindío
Grupo de Estudio en Enfermedades Cardiovasculares y Metabólicas (GECAVYME). Facultad de Ciencias de la Salud, Universidad del Quindío. Programa de Biología. Facultad de Ciencias Básicas y Tecnologías. Universidad del Quindío. Armenia-Colombia.
Sandra Sulay Arango Varela, Instituto Tecnológico Metropolitano de Medellín
Grupo Sinergia. Facultad de Ingeniería, Instituto Tecnológico Metropolitano. Medellín-Colombia.
Patricia Landázuri, Universidad del Quindío
Grupo de Estudio en Enfermedades Cardiovasculares y Metabólicas (GECAVYME). Facultad de Ciencias de la Salud, Universidad del Quindío. Armenia-Colombia.

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Publicado
2013-12-09
Cómo citar
Aguillón Osma, J., Maldonado, M. E., Loango Chamorro, N., Arango Varela, S. S., & Landázuri, P. (2013). Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis. Perspectivas En Nutrición Humana, 15(1), 13-25. Recuperado a partir de https://revistas.udea.edu.co/index.php/nutricion/article/view/17897
Sección
Artículos de Investigación