Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis

  • Johanny Aguillón Osma Universidad del Quindío
  • María Elena Maldonado Universidad de Antioquia
  • Nelsy Loango Chamorro Universidad del Quindío
  • Sandra Sulay Arango Varela Instituto Tecnológico Metropolitano de Medellín
  • Patricia Landázuri Universidad del Quindío
Palabras clave: Passiflora edulis, antioxidante, antiproliferativo, citotóxico


Background: Extracts from a variety of fruit trees have been used for therapeutic applications for preventing oxidative stress associated to chronic diseases. Objective: To investigate the antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits of Passiflora edulis. Materials and methods: A preliminary phytochemical screening was performed; antioxidant activity was evaluated through DPPH assay, the scavenging activity for hydroxyl radical, antihemolytic activity and total phenolic content; cytotoxic and antiproliferative activities were evaluated by MTT and sulforhodamine B assays respectively in colon adenocarcinoma SW480 cells and their metastatic-derived SW620 cells. Results: Phytochemical analyses revealed presence of tannins, flavonoids and cardiotonic glycosides. Ethanolic extract from leaves showed the best antioxidant activity (EC50 = 0.096 mg/ml) in the DPPH assay and the juice (EC50 = 0.022 mg/ml) for the Hydroxyl free radical-scavenging activity. All extracts inhibited more than 98% the hemolysis induced by H2 O2 . The aqueous extract from leaves showed the highest cytotoxic activity against SW480 and SW620 cells. Conclusions: Findings from this study suggest that P. edulis is a potential source of phytochemical compounds with antioxidant and antiproliferative properties.
= 1431 veces | PDF
= 579 veces|


La descarga de datos todavía no está disponible.

Biografía del autor/a

Johanny Aguillón Osma, Universidad del Quindío
Grupo de Estudio en Enfermedades Cardiovasculares y Metabólicas (GECAVYME). Facultad de Ciencias de la Salud, Universidad del Quindío. Armenia-Colombia.
María Elena Maldonado, Universidad de Antioquia
Grupo de Investigación Impacto de los Componentes Alimentarios en la Salud (ICAS). Escuela de Nutrición y Dietética, Universidad de Antioquia. Medellín-Colombia.
Nelsy Loango Chamorro, Universidad del Quindío
Grupo de Estudio en Enfermedades Cardiovasculares y Metabólicas (GECAVYME). Facultad de Ciencias de la Salud, Universidad del Quindío. Programa de Biología. Facultad de Ciencias Básicas y Tecnologías. Universidad del Quindío. Armenia-Colombia.
Sandra Sulay Arango Varela, Instituto Tecnológico Metropolitano de Medellín
Grupo Sinergia. Facultad de Ingeniería, Instituto Tecnológico Metropolitano. Medellín-Colombia.
Patricia Landázuri, Universidad del Quindío
Grupo de Estudio en Enfermedades Cardiovasculares y Metabólicas (GECAVYME). Facultad de Ciencias de la Salud, Universidad del Quindío. Armenia-Colombia.


Dabrowska C, Moya M. Vitaminas y antioxidantes. Madrid: Sanidad y Ediciones; 2009. p.2-34.

Martínez S, González J, Culebras JM, Tuñón MJ. Los flavonoides: propiedades y acciones antioxidante: revisión. Nutr Hosp.2002;17:271-8.

Sokmen M, Angelova M, Krumova E, Pashova S, Ivancheva S, Sokmen A, et al. In vitro antioxidant activity of poliphenol extracts with antiviral properties from Geranium sanguineum L. Life Sci. 2005;76:2981-93.

Posada M, Pineda V, Agudelo G. Los antioxidantes de los alimentos y su relación con las enfermedades crónicas. Perspect Nutr Humana.2002;7:1-22.

Nijveldt R, Nood E, Hoorn D, Boelens P, Norren K, Leeuwen A. Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr. 2001;74:418-25.

Sita SP. Morphology and pharmacology of Passiflora edulis: a review. JHerbal Med Toxicol.2009;3:1-6.

Bum E, Ngah E, Ekoundi C, Dong C, Mbomo R, Rakotonirina S, et al. Sedative and anticonvulsant properties of Passiflora edulis: dried leaves decoction in mice. Afr J Trad Compl Alternative Med.2004;1:63-71.

Kamaldeep D, Sanju D, Anupam S. Passiflora: a review update. J Ethnopharmacol. 2004;94:1-23.

Movafegh A, Alizadeh R, Hajimohamadi F, Esfehani F, Nejatfar M. Preoperative oral Passiflora reduces anxiety in ambulatory surgery patients: A double-blind, placebo-controlled study. Int Anesth Res Soc.2008;106:1728-32.

Santos KC, Tessaro SM, Davet S, Weber M, Monteiro RM, Moraes C. Sedative and anxiolytic effects of methanolic extract from the leaves of Passiflora actinia. Braz Arch Biol Technol. 2006;49:565-73.

Puricelli L, Dell I, Sartor L, Garbisa S, Caniato R. Preliminary evaluation of inhibition of matrix-metalloproteinase MMP-2 and MMP-9 by Passiflora edulis and P. foetida aqueous extracts. Fitoterapia. 2003;74:302-4.

Bilbao M. Análisis fitoquímico preliminar: química de productos naturales. Armenia: Universidad del Quindío; 1997.

Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. Food Sci Technol. 1995;28:25-30.

Gunjan G, Rajkumar R, Ashok K, Lanzar M. Therapeutic potential of polar and non-polar extracts of Cyanthillium cinereum in vitro. Evid Based Compl Alternative Med. 2011;10:1-11.

Nabavi SF, Ebrahimzadeh M, Nabavi S, Eslami B. Antioxidant activity of flower, stem and leaf extracts of Ferula gummosa Boiss. Grasas Aceites. 2011;61:244-50.

Yang G, Wang D, Tang W, Chen X, Fan L, Zhang F, et al. Anti-inflammatory and antioxidant activities of Oxytropis falcate fractions and its possible anti-inflammatory mechanism. Chinese J Nat Med. 2010;8:285-92.

Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal Biochem. 1999;269:337-41.

Maldonado M, Bousserouel S, Gossé F, Minker C, Lobstein A, Raul F. Differential induction of apoptosis by apple procyanidins in trail-sensitive human colon tumor cells and derived trail-resistant metastatic cells. J Cancer Mol. 2009;5:21-30.

Rahman A, Choudhary MI, Thomsen WJ. Bioassay techniques for drug development. Netherlands: Harwood Academic Publishers; 2001. p.34-5.

Gossé F, Guyot S, Roussi S, Lobstein A, Fischer B, Seiler N, et al. Chemopreventive properties of apple procyanidins on human colon cancer-derived metastatic SW620 cells and in a rat model of colon carcinogenesis, Carcinogenesis. 2005;26:1291-5.

Rojas JP. Estudio preclínico y clínico de la seguridad y la actividad antihipertensiva de Passiflora edulis sims (maracuyá). Tesis Doctor en Farmacia y Bioquímica. Lima: Universidad Nacional de San Marcos; 2009.

Sunitha M, Devaki K. Antioxidant activity of Passiflora edulis Sims leaves. Indian J Pharm Sci. 2009;71:310-1.

Vasco C, Ruales J, Kamal-Eldin A. Total phenolic compounds and antioxidant capacities of major fruits from Ecuador. Food Chem. 2008;111:816-23.

Ripa F, Haque M, Nahar L, Islam M. Antibacterial, cytotoxic and antioxidant activity of Passiflora edulis Sims. Eur J Sci Res.2009;31:590-8.

García D, Ojeda F. Evaluación de los principales factores que influyen en la composición fitoquímica de Morus alba (Linn.). II polifenoles totales. Pastos Forrajes. 2004;27:59-64.

Murillo E, Lombo O, Tique M, Méndez J. Potencial antioxidante de Bauhinia kalbreyeri Harms (Fabaceae). Inform Tecnol. 2007;18:65-74.

Rodríguez L, López L, García M. Determinación de la composición química y actividad antioxidante en distintos estados de madurez de frutas de consumo habitual en Colombia, mora (Rubus glaucus B), maracuyá (Passiflora edulis), guayaba (Psidium guajava L) y papayuela (Carica cundinamarcensis). Rev Asoc Col Ciencia Tecnol Alimentos. 2010;21:16-34.

Rudnicki M, Fonseca J, Dal-Pizzol F. Propiedades antioxidantes de los extractos de Passiflora alata Dryander y de Passiflora edulis Sims. Porto Alegre: Universidad Federal Do Rio Grande Do Sul; 2005.

Murcia M, Jiménez A, Martínez M. Evaluation of the antioxidant properties of mediterranean and tropical fruits compared with common food additives. J Food Protec. 2001;64:2037-46.

Ferreres F, Sousa C, Valentao P, Andrade P, Seabra R, Gil A. New C-Deoxyhexosyl flavones and antioxidant properties of Passiflora edulis leaf extract. J Agric Food Chem. 2007;55:10187-93.

Ajila C, Prasada U. Protection against hydrogen peroxide induced oxidative damage in rat erythrocytes by Mangifera indica L.peel extract. Food Chem Toxicol. 2008;46:303-9.

Zhu Q, Holt R, Lazarus S, Orozco T, Keen C. Inhibitory effects of cocoa flavanols and procyanidin oligomers on free radical induced erythrocyte hemolysis. Exp Biol Med. 2002;227:321-9.

Suffness M, Pezzuto JM. Assays related to cancer drug discovery. In: Hostettmann K, ed. Methods in plant biochemistry: assays for bioactivity. London: Academic; 1990. p.71-133.

De Neira CM. The effects of yellos passion fruit, Passiflora edulis Flavicarpa, phytochemicals on cell cycle arrest and apoptosis of leukemia lymphoma MOLT-4 cell line. Thesis for Master Science. Florida: University of Florida; 2003.

Silva D, Freitas A, Barros F, Lins K, Alves A, Alencar N, et al. Polysaccharide isolated from Passiflora edulis: Characterizatio and antitumor properties. Carbohydr Polymer. 2012;87:139-45.

Takai N, Ueda T, Nishida M, Nasu K, Narahara H. Bufalin induces growth inhibition, cell cycle arrest and apoptosis in human endometrial and ovarian cancer cells. Int J Mol Med. 2008;21:637-43.

Yan S, Qu X, Xu C, Zhu Z, Zhang L, Xu L, et al. Down-regulation of Cbl-b by bufalin results in up-regulation of DR4/DR5 and sensitization of TRAIL-induced apoptosis in breast cancer cells. J Cancer Res Clin Oncol. 2012;138:1279-89.

Kawazoe N, Aiuchi T, Masuda Y, Nakajo S, Nakaya K. Induction of apoptosis by bufalin in human tumor cells is associated with a change of intracellular concentration of Na+ ions. J Biochem. 1996;126:278-86.

Zhu Z, Sun H, Ma G, Wang Z, Li E, Liu Y. Bufalin induces lung cancer cell apoptosis via the inhibition of PI3K/Akt pathway. Int J Mol Sci. 2012;13:2025-35.

Perrone A, Capasso A, Festa M, Kemertelidze E, Pizza C, Skhirtladze A, et al. Antiproliferative steroidal glycosides from Digitalis ciliata. Fitoterapia. 2012;83:554-62.

Elbaz H, Stueckle T, Wang Y, O´Doherty G, Lowry D, Sargent L, et al. Digitoxin and a synthetic monosaccharide analog inhibit cell viability in lung cancer cells. Toxicol Appl Pharmacol. 2012;258:51-60.

Rudniki M, Oliveira M, Pereira T, Regginatto F, Pizzol F, Fonseca J. Antioxidant and antiglycation properties of Passiflora edulis extracts. Food Chem. 2007;100:719-24.

Cómo citar
Aguillón Osma, J., Maldonado, M. E., Loango Chamorro, N., Arango Varela, S. S., & Landázuri, P. (2013). Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis. Perspectivas En Nutrición Humana, 15(1), 13-25. Recuperado a partir de
Artículos de Investigación