Effects of all-trans retinoic acid on the production of cytokines by peripheral blood mononuclear cells (PBMC) in children with acute malnutrition

Authors

  • Gloria Cecilia Deossa Restrepo Universidad de Antioquia
  • Claudia María Velásquez Rodríguez Universidad de Antioquia
  • Juliana Orozco Universidad de Antioquia
  • Ángel González Universidad de Antioquia

DOI:

https://doi.org/10.17533/udea.penh.9567

Keywords:

tretinoin, cytokines, protein-energy malnutrition, C-reactive protein, children

Abstract

Objective: To compare the effect of all-trans retinoic acid in the production of cytokines by peripheral blood mononuclear cells (PBMC) among children with moderate malnutrition (Dn-M), severe (Dn-G) and a control group of children eutrophic (Eu). Methodology: Quasi-experimental research in vitro, to assess the effect of different doses of atRA (0, 17, 30 and 50nM) on the concentration of cytokines (L-8, IL-1β, IL-6, IL-10, TNF-α and IL-12p70) in PBMC of 21 children, divided into three groups: seven with moderate malnutrition (Dn-M), 7 with severe malnutrition (Dn-G) and 7 healthy children (Eu). Results: Although 71% of malnourished children (Dn-G and Dn-M) showed signs of infection, had a population of leukocytes and lymphocytes lower than the Eu. Children Dn-G reported a concentration of C-reactive protein (CRP), significantly higher than the other two groups and simultaneously younger, shorter (T/E) and lower hemoglobin. The concentration of IL-1β, IL-6, IL-8, TNFα and IL-10 in PBMC was higher in Dn-M than in Dn-G and Eu. Comparison between groups was found that 50 nM of atRA concentration of IL-8 was significantly lower and the higher IL-10 in Dn-G compared with the other two groups. MANOVA analyses indicate these differences were attributed to the fact to belonging the group (Dn-G) and not the dose of atRA (50 nM). Analysis of the effect of different doses of atRA in each group showed a significant decrease with 50nM of atRA of IL-8 in Dn-G and IL-10 in Eu. IL-12p70 increased with doses of 17 nM in Dn-M. Conclusion: atRA affects the production of cytokines but the main determinant of concentration is the type of malnutrition.
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Author Biographies

Gloria Cecilia Deossa Restrepo, Universidad de Antioquia

School of Nutrition and Dietetics, Universidad de Antioquia. Medellin Colombia.

Claudia María Velásquez Rodríguez, Universidad de Antioquia

Food and Human Nutrition Research Group. School of Nutrition and Dietetics, Universidad de Antioquia. Medellin Colombia.

Juliana Orozco, Universidad de Antioquia

School of Nutrition and Dietetics, Universidad de Antioquia. Medellin Colombia.

Ángel González, Universidad de Antioquia

Group of Medical and Experimental Mycology. Corporation for Biological Research (CIB). Universidad de Antioquia. Medellin Colombia.

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Published

2011-07-10

How to Cite

Deossa Restrepo, G. C., Velásquez Rodríguez, C. M., Orozco, J., & González, Ángel. (2011). Effects of all-trans retinoic acid on the production of cytokines by peripheral blood mononuclear cells (PBMC) in children with acute malnutrition. Perspectivas En Nutrición Humana, 12(2), 119–134. https://doi.org/10.17533/udea.penh.9567

Issue

Vol. 12 No. 2 (2010)

Section

Research

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Derechos de autor 2024 Universidad de Antioquia

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